A Lab Notebook

dromeas - an oceanographic lab notebook and running log
Monday, November 21, 2005
 
AlphaTemp1.7
I monitored thje cultures for John today. I sampled too late in the day run the 18 deg culture through the flocculator. We should do this tomorrow.


 
FlumePost
This is more of a note to myself rather than a general post since I left my notebook downstairs.
Dilution for CC => 5%
pH = 9.25
Fv/Fm = 0.575, Dilution =>0.1/3

Thursday, November 17, 2005
 
AlphaTemp1.5
All of the cultures are in slow decline. Not a serious crash per se, but with Fv/Fm in the high 0.3s, they aren't looking too hot at the moment. Sampling of the cultures on 16 November occurred a bit later than I had hoped. There was considerable difficulty with the other concurrent experiment we are running. I had serious issues with the machine that controls the position of the laser we use to measure particle velocity in the annular flume. As a result, I didn't get a chance to sample from the temperature experiments until 5pm. Fortunately, we are waiting for the Fv/Fm of the cultures to drop below 0.3 to run another flocculator time series.

Wednesday, November 09, 2005
 
AlphaTemp1.2
Started the first day of full scale sampling. The basic numbers are posted below while the goods are kept in secrecy for the killer science paper John and I are planning on writing based on the results of this experiment. In reality however, we started sampling at 1430, John left at 5:45 for class, and I finished around 1930. We used a 100um orifice on the Coulter Counter, and may have to use a larger orifice in the coming days. We filtered 6x60ml from each culture, 3=>chl-a, 3=>TEP/Alcian Blue.

Tuesday, November 08, 2005
 
AlphaTemp1.1
The basic numbers from today. We are conducting an experiment to test the coagulation efficiency, or stickiness, of Skeletonema at various temperatures. We are using the efficiency of PSII as an indication of culture physiology. The stickiness of each culture at a given physiological state will be compared. Today, the efficiency of PSII was less than our sampling threshold of 0.2. No sampling of the cultures today. The spreadsheet has been moved to the most recent day of the experiment.


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