This is an online lab notebook to document my research at the Institute of Marine at Coastal Sciences at Rutgers University. And by popular demand, a running log.
About Me
- Name: alex
- Location: Rutgers University, New Jersey, United States
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dromeas - an oceanographic lab notebook and running log
Monday, April 11, 2005
Contaminated Cultures... Start Again!
Monday April 12
Both of my 20 liter cultures are in stationary phase. One of the cultures has become contaminated by a smaller species (coulter counter and visual observation). Additional evidence for contamination was the increased Fv/Fm of the suspended culture media in the contaminated culture. The non-contaminated culture had the expected low Fv/Fm of suspended media associated with stationary phase cultures which have mostly settled to the bottom.
I autoclaved enough of each of the f/2 solution ingredients to fill 2 new 20 liter culture vessels and begin again.
This contamination has set back my plans to innoculate a flume for a big experiment.
I filtered an resuspended the non-contaminated culture in 1 liter of media. I also took a sample of the uncontaminated culture and used it to innoculate another 1 liter culture. I am now growing 2 separate 1 liter cultures of S. costatum.
Hopefully there is a picture below of me working hard deploying a glider about 2 weeks ago.
Also, I ran at bear Mountain State Park in NY this weekend. There are a couple of small peaks near 1200 feet nestled along the banks of the Hudson (elevation ~ 100 feet). There are a few roads of varying length that one can take to get the 1000 foot elevation gain. I will be back this weekend to continue strength training.
- posted by alex @ 18:01 1 comments
